Perkins Lab
Single Molecule Biophysics

Papers

T. T. Perkins, "Steady-state gain and saturation flux measurements in a high efficiency, electron-beam-pumped, Ar-Xe laser," J. Appl. Phys. 74, 4860 (1993).
T. T. Perkins, S. R. Quake, D. E. Smith, S. Chu, "Relaxation of a Single DNA Molecule Observed by Optical Microscopy," Science 264, 822 (1994).
T. T. Perkins, D. E. Smith, S. Chu, "Direct Observation of Tube-like Motion of a Single Polymer Chain," Science 264, 819 (1994).
T.T. Perkins, D. E. Smith, S. Chu, "Optical Manipulation of Single DNA Molecules," Physics in 1994 (AIP)
T. T. Perkins, D. E. Smith, R. G. Larson, S. Chu, "Stretching of a Single Tethered Polymer in a Uniform," Science 268, 83 (1995).
D. E. Smith, T. T. Perkins, S. Chu, "Self Diffusion of an Entangled DNA Molecule by Reptation," Phys. Rev. Lett. 75, 4146 (1995).
D. E. Smith, T. T. Perkins, S. Chu, "Dynamical Scaling of DNA Diffusion Coefficients," Macromolecules 29, 1372 (1996).
R. G. Larson, T. T. Perkins, D. E. Smith, S. Chu, "Hydrodynamics of DNA Molecule in a Flow Field," Phys. Rev. E. 55, 1794 (1997).
T. T. Perkins, D. E. Smith, S. Chu, "Single Polymers in Elongational Flow," Science 276, 2016 (1997).
R. G. Larson, T. T. Perkins, D. E. Smith, S. Chu, "Hydrodynamics of Flow Past a DNA Molecule," Flexible Polymer Chains in Elongational Flow: Theories and Experiments, Eds. T.Q. Nguyen, H. H. Kausch, (Springer-Verlag) (1999).
T. T. Perkins, D. E. Smith, S. Chu, "Single Polymers in Elongational Flows: dynamic, steady-state, and population averaged properties," Flexible Polymer Chains in Elongational Flow: Theories and Experiments, Eds. T.Q. Nguyen, H. H. Kausch, (Springer-Verlag) (1999).
T. T. Perkins, R.V. Dalal, P. M. Mitsis, S.M. Block, "Sequence Dependent Pause of Single Lambda exonuclease molecules," Science 301 1914 (2003) published online Aug 28, 2003, 10.1126/science.1088047
T. T. Perkins, H.-W. Li, R. V. Dalal, J. Gelles, S. M. Block, "Forward and Reverse Motion of Single RecBCD Molecules on DNA," Biophys. J. 86, 1640 (2004).
L. Nugent-Glandorf & T. T. Perkins, "Measuring 0.1-nm motion in 1 ms in an optical microscope with differential backfocal plan detection," Optics Letters, 29, 2611 (2004). Highlighted as an Editor's Choice is Science, 306, 1862 (2004).
Y. Seol, A. E. Carpenter, T. T. Perkins, "Gold nanoparticles: enhanced optical trapping and sensitivity coupled with significant heating," Optics Letters, 31, 2429 (2006).
A. R. Carter, G. M. King, T. A. Ulrich, W. Halsey, D. Alchenberger, T. T. Perkins, "Stabilization of an optical microscope to 0.1 nm in 3D," Applied Optics, 46, 421 (2007).
A. R. Carter, G. M. King, T. T. Perkins, "Back-scattered detection provides atomic-scale localization precision, stability, and registration in 3D," Optics Express, 15, 13434 (2007).
Y. Seol, J. Li, P. C. Nelson, T. T. Perkins, M. D. Betterton, "Elasticity of Short DNA molecules: theory and experiment for contour lengths of 0.6-7 µm," Biophysical Journal, (2007). New and Notable
A. R. Hieb, W. A. Halsey, M. D. Betterton, T. T. Perkins, J. F. Kugel1, J. A. Goodrich, "TFIIA Changes the Conformation of the DNA in TBP/TATA Complexes and Increases their Kinetic Stability," Journal of Molecular Biology, 372, 619 (2007).

Thesis


T.T. Perkins, "Exploring polymer dynamics with single DNA moecules" (Thesis)
Packages and Overnight Shipping Address
Thomas Perkins
JILA, Room S-175
University of Colorado at Boulder
Boulder, CO 80309

US Postal Address
Thomas Perkins
JILA, 440 UCB
University of Colorado at Boulder
Boulder, CO 80309-0440