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Advanced Optical Microscopies for Cell Bioimaging

Event Details

Event Dates: 

Thursday, January 12, 2017 - 4:00pm

Seminar Location: 

  • JILA X317

Speaker Name(s): 

Shengwang Du

Speaker Affiliation(s): 

Hong Kong University of Science and Technology
Seminar Type/Subject

Scientific Seminar Type: 

  • JILA Public Seminar

Event Details & Abstract: 

Reception in the Hbar at 3:30 pm

The most popularly used optical microscope in cell bioimaging is the confocal microscope developed in the 20th century. However, as a traditional optical-wave microscope, the confocal microscopy suffers from the diffraction-limited imaging resolution (>=200 nm for visible light) and the severe phototoxicity that limits its application not suitable for live-cell imaging. In this talk, I will review the recent development of two advanced optical microscopy techniques in my group to overcome these two limitations: super-resolution microscopy and light-sheet microscopy. We have developed a user-friendly two(three)-color super-resolution localization microscope with a resolution <20 nm. For live-cell imaging, we have invented a 5D (3D space + 1D time + 1D color) line Bessel Sheet (LBS) microscope with lateral/axial resolution of 250 nm/350 nm, and 2 Hz temporal resolution per channel for a 3D volume of 30 umÍ30 umÍ30 um. Both microscopy technologies have been successfully commercialized and released to market. The works were supported by a grant from the Offices of the Provost, VPRG and Dean of Science, HKUST (HKUST VPRGO12SC02), and the Hong Kong Research Grants Council (Project Nos. HKUST12/CRF/13G, C6030-14E).